Saposin C (SAP-C) is a small, heat-stable lysosomal glycoprotein derived from the prosaposin precursor protein via site-specific proteolytic cleavage. Among the four prosaposin-derived domains, Saposin C shares the highest structural and sequence homology with Saposin A. It acts as an indispensable biological cofactor required for the lysosomal activation of β-glucocerebrosidase (GBA), the enzyme deficient in Gaucher disease. Additionally, Saposin C plays a critical role in cellular immunology by facilitating the presentation of lipid antigens through CD1b molecules to human T cells. While native lysosomal saposins are heavily glycosylated, non-glycosylated recombinant variants expressed in bacterial systems maintain full biological activation properties in functional in vitro biochemical assays.

Product Features & Specifications

• Expression System: Engineered and expressed in an optimized Escherichia coli (E. coli) bacterial host.
• Fusion Tag: Features a C-terminal Polyhistidine (6xHis) tag to allow robust detection, tracking, and oriented immobilization.
• High Purity: Verified at >90% purity by Coomassie blue staining on SDS-PAGE.
• Formulation & Concentration: Supplied at a concentration of 1.0 mg/mL (determined by A280) in 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 50% Glycerol for premium structural stability.

Validated Assay Applications

This recombinant human Saposin C construct is specifically optimized for lysosomal enzyme activity assays, in vitro glucocerebrosidase reconstitution kinetics, lipid-binding biophysical studies, CD1b lipid antigen presentation assays, Surface Plasmon Resonance (SPR), and Bio-Layer Interferometry (BLI).

Catalog Numbers: SAPC-301-1 (0.1 mg) | SAPC-301-2 (1.0 mg). This product is intended for laboratory research use only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.