King Test Rapid One Step hCG Midstream Pregnancy Test Kit is the self performing immunoassay designed for the determination of pregnancy at home. King Test Rapid One Step HCG Midstream Pregnancy Test Kit detects the hCG (Human Chorionic Gonadotropin) hormone that appears in the urine of a pregnant woman from the first days of conception. In pregnancy, hCG is produced first by the fertilized egg and later the placenta. A fertilized egg typically implants into the lining of the uterus (womb) 6-8 days before the missed period, when tiny amounts of hCG start to appear in the mother’s blood and urine. Though hCG levels are very low at first, the level rises rapidly and predictably in the first weeks of pregnancy. The earliest you can test with the most sensitive tests currently available is 6 days before your missed period (typically 10 days after ovulation). King Test Rapid One Step Pregnancy Test Midstream gives accurate and clear results for the possibility of pregnancy. If only one pink line appear in the control area, you can assume that you are not pregnant. If two pink lines appear both in the control and test area, you can assume that you are pregnant. Simply, one line means negative, while two lines means you are pregnant. If there is no distinct pink-purple colored band visible both in the control and test area, the test is invalid. One pink line appear in the test area is also considered as invalid test. It is recommended to repeat the test in case invalid results.