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Amid Biosciences HRV 3C protease is a recombinant cysteine protease derived from human Rhinovirus type 14. The enzyme is known as PreScission Protease. HRV 3C protease specifically cleaves between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro. Besides the recognition sequence, the secondary and tertiary structures of the fusion protein influence on substrate recognition and cleavage.
The recombinant enzyme is a 21 kDa protein with polyhistidine (6xHis) tag, so it can be removed from the protease reaction solution through immobilized metal affinity chromatography (IMAC).
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