High-Efficiency SS320 Competent Cells for Phage Display

The E. coli strain SS320 (also known as MC1061F′) is a non-amber suppressor strain prepared as high transformation efficiency (≥ 1 × 10¹⁰ cfu/μg) electrocompetent cells. It is specifically optimized for robust phage display library screening and constructing large antibody or protein libraries.

Key Features & Benefits

• Exceptional Efficiency: > 1 x 10¹⁰ transformants / µg pBR322, perfect for large-scale antibody phage display.
• F′ Pilus Maintenance: Harbors an episome containing the F′ pilus required for bacteriophage entry, allowing seamless amplification of phage libraries.
• Tetracycline Resistant: Contains the Tn10 (tetR) gene; cells should be grown with tetracycline to maintain the F′ pilus.
• Fast-Growing: Accelerates biopanning and cloning workflows.

Product Specifications

• Genotype: hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galU galK rpsL thi [F´ proAB+ lacIq lacZΔM15 Tn10 (tetr)]
• Applications: Peptide phage display, antibody library preparation, biopanning.
• Storage: Store at -80°C. Ships on dry ice.

Intended for Research Use Only (RUO).