Neurocan Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65654-50, BS65654-100

Swiss-Prot: P55066

Host: Rabbit

Neurocan Polyclonal Antibody-BS65654

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22kD each. Later neurocan comprises a 180kD core protein Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neural damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration. Alternative names: Chondroitin sulfate proteoglycan 3; Cspg3; NCAN; NEUR; Neurocan core protein.

Purification and Purity: affinity purified by Protein A

Specificity: Neurocan Polyclonal Antibody detects endogenous levels of Neurocan protein.

Bioworld Molecular Weight: 140kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Positive sample：Paraformaldehyde-fixed, paraffin embedded (mouse brain)Negative sample：Paraformaldehyde-fixed, paraffin embedded (mouse liver)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurocan) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C,, Positive sample：Paraformaldehyde-fixed, paraffin embedded (rat brain)Negative sample：Paraformaldehyde-fixed, paraffin embedded (mouse liver)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurocan) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Alternative Name: Chondroitin sulfate proteoglycan 3; Cspg3; NCAN; NEUR; Neurocan core protein; NCAN_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human Neurocan

Modification: Unmodified

Conjugate: Unconjugated