GAPDH (Loading Control) Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65656-50, BS65656-100

Swiss-Prot: P04406

Host: Rabbit

GAPDH (Loading Control) Polyclonal Antibody-BS65656

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat,

Applications: WB, IHC-P，ICC,

All Applications: WB=1:5000-20000 IHC-P=1:100-500

Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

Purification and Purity: affinity purified by Protein A

Specificity: GAPDH Polyclonal Antibody detects endogenous levels of GAPDH protein.

Bioworld Molecular Weight: 38kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample: 293T (human) cell lysate at 30ug;
Primary:
Lane 1: Anti-GAPDH at 1/2000 dilution
Lane 2: Anti-GAPDH at 1/10000 dilution
Lane 3: Anti-GAPDH at 1/40000 dilution
Lane 4: Anti-GAPDH at 1/80000 dilution, Sample:
293T (Human) Lysate at 40 ug
Primary:
Anti-GAPDH at 1/2000~1/20000 dilution

Alternative Name: 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.

Immunogen: Recombinant human GAPDH full length protein

Modification: Unmodified

Conjugate: Unconjugated