Nanog Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS70530-50, BS70530-100

Swiss-Prot: Q9H9S0

Host: Rabbit

Nanog Polyclonal Antibody-BS70530

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:1000 - 1:2000 | IHC,1:50 - 1:200

Background: The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 48kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of NIH/3T3 cells, using Nanog antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s., Immunohistochemistry of paraffin-embedded Human testicular seminoma using Nanog Rabbit pAb at dilution of 1:25.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: NANOG

Immunogen: Recombinant fusion protein of human Nanog(NP_079141.2).

Modification: Unmodified

Conjugate: Unconjugated