Ras Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91158-50, BS91158-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01111(Human) P01112(Human) P01116(Human) P08556(Mouse) P32883(Mouse) Q61411(Mouse) P08644(Rat) P20171(Rat) Q04970(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, zebrafish

Applications: WB, ICC/IF, IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500

Background: The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Ras polyclonal antibody detects endogenous levels of Ras protein.

Bioworld Molecular Weight: 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: MCF-7
Lane 3: Hela
Lane 4: zebrafish, ICC staining Ras in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: NRAS, HRAS, KRAS, C-BAS/HAS, c-H-ras, C-HA-RAS1, CTLO, GTPase HRas, GTPase KRas, GTPase NRas, H-Ras-1, H-RASIDX, Ha-Ras, HAMSV, HRAS, HRAS1, K RAS2A, K RAS2B, K RAS4A, K RAS4B, K-RAS, KRAS, KRAS1, KRAS2, N-RAS, N-terminally processed, NRAS, NRAS1, p21ras, RASH, RASH1, RASK2, Transforming protein p21, v Ha ras Harvey rat sarcoma viral oncogene homolog, v Ki ras2 Kirsten rat sarcoma viral oncogene homolog, v ras neuroblastoma RAS viral oncogene homolog,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified