CHRNA10 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65348-50, BS65348-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q9GZZ6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ELISA

All Applications: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Background: function:Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.,miscellaneous:The hetero-oligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).,similarity:Belongs to the ligand-gated ionic channel (TC 1.A.9) family.,subunit:Forms hetero-oligomeric channels in conjunction with CHRNA9. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 hetero-oligomers.,tissue specificity:Expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes.,

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: AChRα10 Polyclonal Antibody detects endogenous levels of AChRα10 protein.

Bioworld Molecular Weight: ~50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of various cells using AChRα10 Polyclonal Antibody, Western Blot analysis of A549 cells using AChRα10 Polyclonal Antibody, Western blot analysis of lysates from HeLa, 293, COLO, and A549 cells, using CHRNA10 Antibody. The Lane on the right is blocked with the synthesized peptide.

Alternative Name: CHRNA10; NACHRA10; Neuronal acetylcholine receptor subunit alpha-10; Nicotinic acetylcholine receptor subunit alpha-10; NACHR alpha-10

Immunogen: The antiserum was produced against synthesized peptide derived from human CHRNA10. AA range:394-443

Conjugate: Unconjugated

Modification: Unmodified