TOK-1 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES6850-50, ES6850-100

Citations, Manuals and MSDS Available upon request.

Background: This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008],

Alternate Name: BCCIP; TOK1; BRCA2 and CDKN1A-interacting protein; P21- and CDK-associated protein 1; Protein TOK-1

Source: Rabbit

Applications: WB; ELISA

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Reactivity: Human; Rat; Mouse;

Immunogen: Synthesized peptide derived from TOK-1 . at AA range: 60-140

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 35kD

Human Gene ID: 56647

Human SWISS Prot NO: Q9P287

Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle pole. Colocalizes with BRCA2 in discrete nuclear foci (PubMed:15713648). In interphase, preferential localizes to the mother centriole (PubMed:28394342). Recruited to the spindle pole matrix and centrosome by microtubules and dynein/dynactin activity (PubMed:28394342).; [Isoform 1]: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Isoform 1/beta tends to be less abundant at, and less strongly associated with, centrosomes than isoform 2/alpha.; [Isoform 2]: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Isoform 2/alpha tends to be more abundant

Research Use Only