LDS-751 is a nucleic acid stain principally detecting DNA. LDS-751 has high affinity for DNA and undergoes fluorescence enhancement upon binding, but with maximal emission at 670 nm. LDS-751 and Thiazole orange are used to differentiate erythrocytes, platelets, reticulocytes, and nucleated cells, and both can be excited at 488 nm.

Product information

CAS Number: 181885-68-7

Molecular Weight: 471.98

Formula: C25H30ClN3O4

Chemical Name: 6-(dimethylamino)-2-[(1E,3E)-4-[4-(dimethylamino)phenyl]buta-1,3-dien-1-yl]-1-ethylquinolin-1-ium perchlorate

Smiles: CC[N+]1=C2C=CC(=CC2=CC=C1/C=C/C=C/C1C=CC(=CC=1)N(C)C)N(C)C.[O-]Cl(=O)(=O)=O

InChiKey: FGBAVQUHSKYMTC-UHFFFAOYSA-M

InChi: InChI=1S/C25H30N3.ClHO4/c1-6-28-23(16-13-21-19-24(27(4)5)17-18-25(21)28)10-8-7-9-20-11-14-22(15-12-20)26(2)3;2-1(3,4)5/h7-19H,6H2,1-5H3;(H,2,3,4,5)/q+1;/p-1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 83.33 mg/mL (176.55 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

The LDS-751 permits the discrimination of intact cells from residual erythrocyte ghosts, platelets and damaged nucleated cells. The forward and orthogonal fight scattering signals of the intact cells, identified by LDS-751 staining allow a clear separation between the major leukocyte populations since the damaged nucleated cells, erythrocytes, erythrocyte cell ghosts and platelets are removed from the display.

Products are for research use only. Not for human use.