Cleaved PARP Mouse mAb

Sizes: 50μL, 100μL

Catalogue Numbers: EM1145-50, EM1145-100

Citations, Manuals and MSDS Available upon request.

Background: Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

Alternate Name: PARP-1, Poly (ADP ribose) polymerase 1, sPARP1, ADPRT1, ADP ribosyltransferase NAD (+)

Source: Mouse

Applications: WB, IHC

Dilution: WB 1:2, 000-5, 000  IHC  1:200-500

Reactivity: Human

Immunogen: Synthetic Peptide

Storage and Stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Clonality: Monoclonal

Isotype: IgG1

Concentration: 1mg/mL

Observed Band (KD): 116, 89kDa

Human Gene ID: 142

Human SWISS Prot NO: P09874

Research Use Only