BBS1 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65686-50, BS65686-100

Swiss-Prot: Q8NFJ9

Host: Rabbit

BBS1 Polyclonal Antibody-BS65686

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep,

Applications: WB, IHC-P

All Applications: WB=1:500-2000 IHC-P=1:100-500

Background: The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.

Purification and Purity: affinity purified by Protein A

Specificity: BBS1 Polyclonal Antibody detects endogenous levels of BBS1 protein.

Bioworld Molecular Weight: 65 kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
HepG2 (Human) Cell Lysate at 30 ug
U2os (Human) Cell Lysate at 30 ug
Primary: Anti- BBS1 at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (mouse brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BBS1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Alternative Name: AI451249; Bardet-Biedl syndrome 1; Bardet-Biedl syndrome 1 homolog; Bardet-Biedl syndrome 1 protein; BBS1; BBS1_HUMAN; BBS2-like protein 2; D19Ertd609e.

Immunogen: KLH conjugated synthetic peptide derived from human BBS5

Modification: Unmodified

Conjugate: Unconjugated