Glutaminase (GLS) Activity Assay Kit

Size: 96T

Catalogue Number: E-BC-K660-M-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: GLS

Research Areas: Amino Acids and Proteins

Result Type: Enzyme Activity

Detection Instrument: Microplate reader

Tested Sample Types: serum (plasma) , animal, plant tissue

Assay Time: 80min

Detection Type: Colorimetric method

Sensitivity: 0.003 U/L

Detection Range: 0.003-18.0 U/L

Test Principle: Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm.

Inter CV: 8.3

Intra CV: 5

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag