25-OH-D (25 Hydroxy Vitamin D) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9387-48, ELK9387-96, ELK9387-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 25 Hydroxy Vitamin D;

Assay Type: Competitive Inhibition

Sensitivity: 0.9 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Reproductive science; Genetic science; Nutrition metabolism; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only