Mouse LEP (Leptin) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-M0037-24, E-CL-M0037-48, E-CL-M0037-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: LEP

UNIProt ID: P41160

Target Synonyms: LEPD; OB; OBS; Obesity Homolog;

Research Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells

Target Species: Mouse

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse LEP concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Mouse LEP in samples. No significant cross-reactivity or interference between Mouse LEP and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag