PCK2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91035-50, BS91035-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16822(Human) Q8BH04(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:100
IHC:1:50-1:200
FC:1:50-1:100

Background: Normal adjustment to changes in blood glucose levels depends on Insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while Insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PCK2 polyclonal antibody detects endogenous levels of PCK2 protein.

Bioworld Molecular Weight: 71 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution., ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PEPCK-M, PCK2, PEPCK2, EC 4.1.1.32, GTP mitochondrial precursor, HGNC:8725, mitochondrial, Mitochondrial phosphoenolpyruvate carboxykinase 2, OTTHUMP00000164700, PCK2, PCKGM, PE, PEP carboxykinase, PEPCK, PEPCK deficiency mitochondrial, PEPCK M, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase [GTP], Phosphoenolpyruvate carboxykinase 2 (mitochondrial), Phosphoenolpyruvate carboxykinase 2 mitochondrial, Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified