HSP90β Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90672-50, BS90672-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08238(Human) P11499(Mouse) P34058(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: HSP90β polyclonal antibody detects endogenous levels of HSP90β protein.

Bioworld Molecular Weight: 83 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Hsp90 beta on different lysates using anti-Hsp90 beta antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: K562, ICC staining Hsp90 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HSP 90, HSP 84, HSP90AB1, 90 kda heat shock protein beta HSP90 beta, D6S182, FLJ26984, Heat shock 84 kDa, Heat shock 90kD protein 1, beta, Heat shock 90kDa protein 1 beta, Heat shock protein 90 alpha family class B member 1, Heat shock protein 90 kDa, Heat shock protein 90kDa alpha (cytosolic) class B member 1, Heat shock protein 90kDa alpha family class B member 1, Heat shock protein beta, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta, HS90B, HSP 84, HSP 90, HSP 90 b, HSP 90b, HSP84, HSP90 BETA, hsp90ab1, HSP90B, HSPC2, HSPCB,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified