MEK Kinase-4 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES6230-50, ES6230-100

Citations, Manuals and MSDS Available upon request.

Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6

Alternate Name: MAP3K4; KIAA0213; MAPKKK4; MEKK4; MTK1; Mitogen-activated protein kinase kinase kinase 4; MAP three kinase 1; MAPK/ERK kinase kinase 4; MEK kinase 4; MEKK 4

Source: Rabbit

Applications: IHC; IF; ELISA

Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Reactivity: Human; Mouse

Immunogen: The antiserum was produced against synthesized peptide derived from human MAP3K4. AA range:1281-1330

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Human Gene ID: 4216

Human SWISS Prot NO: Q9Y6R4

Subcellular Location: Cytoplasm, perinuclear region. Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.

Research Use Only