Hexokinase (HK) Activity Assay Kit

Size: 96T

Catalogue Number: E-BC-K610-M-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: HK

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Enzyme Activity

Detection Instrument: Microplate reader

Tested Sample Types: animal tissue, cell

Assay Time: 30 min

Detection Type: Colorimetric method

Sensitivity: 5.93 U/L

Detection Range: 5.93-40.11 U/L

Test Principle: HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.

Inter CV: 9.1

Intra CV: 3.3

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag