Caspase-1 p20 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65650-50, BS65650-100

Swiss-Prot: P29466

Host: Rabbit

Caspase-1 p20 Polyclonal Antibody-BS65650

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF ICC ELISA

All Applications: WB 1:500 - 1:2000
IHC 1:100 - 1:500
IF 1:100 - 1:500
ICC 1:100 - 1:500
ELISA 1:5000 - 1:10000

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq].

Purification and Purity: affinity purified by Protein A

Specificity: Caspase-1 p20 Polyclonal Antibody detects endogenous levels of Caspase-1 p20 protein.

Bioworld Molecular Weight: ~ 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary:
Anti-Caspase-1 p20 at 1/1000 dilution., Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃.

Alternative Name: caspase-1 isoform alpha p20 subunit; CASP1; ICE; IL1BC; Caspase-1 subunit p20; CASP 1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45

Immunogen: KLH conjugated synthetic peptide derived from mouse Caspase-1 p20

Modification: Unmodified

Conjugate: Unconjugated