Occludin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90980-50, BS90980-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16625(Human) Q61146(Mouse) Q6P6T5(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, FC

All Applications: WB:1:1,000
FC:1:50-1:100

Background: Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Flow cytometric analysis of 293 cells with Occludin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as theSecondary antibody.

Bioworld Molecular Weight: 59 kDa

Specificity: Occludin polyclonal antibody detects endogenous levels of Occludin protein.

Alternative Name: OCLN, BLCPMG, FLJ08163, FLJ18079, FLJ77961, FLJ94056, MGC34277, Occludin, Ocln, OCLN, Tight junction protein occludin,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified