14-3-3 β (Q69) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2176-50, BS2176-100

Sizes: 50µl, 100µl

Swiss-Prot: P31946

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: 14-3-3 β (Q69) polyclonal antibody detects endogenous levels of 14-3-3 β protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of 14-3-3 β (Q69) polyclonal antibody at 1:500 dilution
Lane 1: U-87MG whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: K562 whole cell lysate (40ug)
Lane 4: HepG2 whole cell lysate (40ug)
Lane 5: HEK293T whole cell lysate (40ug)
Lane 6: PC12 whole cell lysate (40ug)
Lane 7: CT-26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of 14-3-3 β (Q69) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 28 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; YWHAB; 14-3-3β; 14-3-3 α; 14-3-3α; 1433B; 14-3-3 β/α; 14-3-3β/α;

Immunogen: Synthetic peptide, corresponding to amino acids 40-90 of Human 14-3-3 β.

Conjugate: Unconjugated

Modification: Unmodified