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BL21(_DE3) _serB is an engineered strain for specific co-translational O-phospho-L-serine Êincorporation in a protein by anÊEscherichia coli. To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21(DE3).
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