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Klenow DNA PolymeraseÊ(3_ _ 5_ exo-) is the large fragment of E. coli DNA polymerase I. It exhibits 5'_3' polymerase activity, but lacks the 3'_5' (proofreading) and 5'_3' (nick-translation) exonuclease activities of DNA Polymerase I. The 3'_5' exonuclease activity of the enzyme is eliminated by mutations in the 3'_5'-exonuclease active site (D355A and E357A ). Polymerase incorporatesÊmodified nucleotides (e.g., fluorescein-, biotin-labeled nucleotides).
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