PRP19 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91118-50, BS91118-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UMS4(Human) Q99KP6(Mouse) Q9JMJ4(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5' splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PRP19 polyclonal antibody detects endogenous levels of PRP19 protein.

Bioworld Molecular Weight: 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution., ICC staining PRP19 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: hPso4, PRPF19, hPso4, NMP200, Nuclear matrix protein 200, Nuclear matrix protein NMP200 related to splicing factor PRP19, pre mRNA processing factor 19, Pre-mRNA-processing factor 19, PRP19, PRP19/PSO4 homolog, PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae), PRP19, PRPF19, PSO4, psoralen 4, Senescence evasion factor, SNEV, UBOX4,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified