RACK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91143-50, BS91143-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P63244(Human) P63245(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC FC

All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200
FC:1:50-1:100

Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. Receptor for activated C kinases, termed RACKs, are intracellular receptors for activated PKC that may be involved in the activation-induced translocation of PKC. RACK1 (receptor for activated C kinase 1) is a 317 amino acid G protein b subunit-like protein that functions as a RACK and inhibits the activity of Src tyrosine kinases. In response to PKC activation, the intracellular localization of RACK1 and PKC bII changes, and RACK1 and PKC bII co-localize to the same sites. RACK1 is therefore thought to be a shuttling protein for PKC bII. A deficit in RACK1 may be associated with impaired PKC activation in the aging brain. The RACK1 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.pombe, S.cerevisiae, K.lactis, E.gossypii, M.grisea, N.crassa, A.thaliana, rice and P.falciparum, and maps to human chromosome 5q35.3.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: RACK1 polyclonal antibody detects endogenous levels of RACK1 protein.

Bioworld Molecular Weight: 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RACK1 on different lysates using anti-RACK1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: SH-SY-5Y
Lane 2: Rat small intestine, Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-RACK1 antibody. Counter stained with hematoxylin.

Alternative Name: HLC-7, RACK1, GNB2L1, Cell proliferation-inducing gene 21 protein, GBLP, Gnb2-rs1, Gnb2l1, Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1, Guanine nucleotide binding protein beta polypeptide 2 like 1, Guanine nucleotide binding protein beta subunit 2 like 1, Guanine nucleotide binding protein beta subunit like protein 12.3, Guanine nucleotide binding protein subunit beta 2 like 1, Guanine nucleotide binding protein subunit beta like protein 12.3, Guanine nucleotide-binding protein subunit beta-2-like 1, Guanine nucleotide-binding protein subunit beta-like protein 12.3, H12.3, HLC-7, Human lung cancer oncogene 7 protein, lung cancer oncogene 7, OTTHUMP00000223704, OTTHUMP00000223870, OTTHUMP00000223891, OTTHUMP00000223893, OTTHUMP00000223900, OTTHUMP00000223902, OTTHUMP00000223930, OTTHUMP00000223931, PIG21, Proliferation inducing gene 21, Protein homologous to chicken B complex protein guanine nucleotide binding, RACK1, Receptor for activated C kinase 1, Receptor for activated C kinase, Receptor of activated protein kinase C 1,

Immunogen: Recombinant protein corresponding to C-terminal human RACK1.

Conjugate: Unconjugated

Modification: Unmodified