Septin 6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS5925-50, BS5925-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14141

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Septin 6, also known as SEPT6, is a 434 amino acid protein that belongs to the septin family. The highly conserved septin family of GTP-binding cytoskeletal proteins is implicated in membrane transport, apoptosis, cell polarity, cell cycle regulation, cytokinesis and other cellular functions. Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. Filaments are assembled from asymmetrical heterotrimers, composed of Septin 2, Septin 6 and Septin 7 that associate head-to-head to form a hexameric unit, however interaction with Septin 12 alters filament structure. Septin 6 shares 40% identity with CDC10 and contains an ATP/GTP-binding site motif. The Septin 6 protein demonstrates nearly ubiquitous expression, with highest level in thymus. The Septin 6 protein is phosphorylated upon DNA damage, probably by Atm or ATR.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Septin 6 polyclonal antibody detects endogenous levels of Septin 6 protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Septin 6 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of Septin 6 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Septin 6 pAb in paraffin-embedded human liver carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Septin6; Septin-6; SEPT6; KIAA0128; SEP2

Immunogen: Synthetic peptide, corresponding to amino acids 267-312 of Human Septin 6.

Conjugate: Unconjugated

Modification: Unmodified