Mouse D-LDH (D-LactateDehydrogenase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9609-48, ELK9609-96, ELK9609-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: DLD; LDHD; Probable D-lactate dehydrogenase; mitochondrial;

Assay Type: Sandwich

Sensitivity: 2.74 mIU/mL

Standard: 300 mIU/mL

Detection Range: 4.69-300 mIU/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal Transduction; Cancer; Kits/ Lysates/ Other; Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse D-LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse D-LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse D-LDH in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only