ADCY 1 (D252) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2391-50, BS2391-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q08828

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Gα s) for GTP, the dissociation of GTP-Gα s from Gβγ and Gα s)-mediated activation of adenylyl cyclase. Adenylyl cyclases type I (AC I) and III (AC III) have distinct staining within the cell nucleus of rat brain sensory neurons. A cyclase I, also known as AC1 or ADCY1, is a 1,119 amino acid multi-pass membrane protein expressed in the brain, retina and adrenal medulla. A cyclase I binds two magnesium ions per subunit and may be involved in regulatory processes in the central nervous system.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ADCY 1 (D252) polyclonal antibody detects endogenous levels of Adenylate cyclase type 1 protein.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ADCY 1 (D252) Antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ADCY 1 (D252) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ADCY 1 (D252) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Adenylate cyclase type 1; ATP pyrophosphate-lyase 1; Adenylate cyclase type I; Adenylyl cyclase 1; Ca(2+)/calmodulin-activated adenylyl cyclase; ADCY1; A Cyclase I

Immunogen: Synthetic peptide, corresponding to amino acids 230-280 of Human ADCY 1.

Conjugate: Unconjugated

Modification: Unmodified