IFN-γ (Q69) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3486-50, BS3486-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01579

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200

Background: Interferon (IFN)-γ is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-γ production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-γ is inducible by IL-2, FGF and EGF. The active form of IFN-γ is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-γ is stabilized by non-covalent interactions through the interface of the helices. IFN-γ tranlsated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IFN-γ (Q69) polyclonal antibody detects endogenous levels of IFN-γ protein.

Bioworld Molecular Weight: ~ 19 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IFN-γ (Q69) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of IFN-γ (Q69) pAb in paraffin-embedded human lymph node tissue., Immunoprecipitation of the Spleen tissue lysate of Rat using IFN-γ (Q69) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using IFN-γ (Q69) pAb #BS3486. “↑”(supernatant); “↓”(deposition)

Alternative Name: IFN-gamma; Immune interferon; IFN-gamma . Interferon gamma; IFNG; IFNγ

Immunogen: Synthetic peptide, corresponding to amino acids 31-80 of Human IFN-γ.

Conjugate: Unconjugated

Modification: Unmodified