PPP1CB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91102-50, BS91102-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62140 Human;P62141 Mouse;P62142 Rat

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, IHC

All Applications: WB:1:1,000-1:10,000
IP:1:50
IHC:1:50-1:200

Background: In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP1 family is comprised of subfamily members PP1α, PP1β and PP1γ, which are MgATP-dependent enzymes. PP1 inactivity is maintained through its association with the inhibitory protein NIPP-1 (nuclear inhibitor of PP1). Phosphorylation of NIPP-1 by cAMP-PK or casein kinase II results in the release of active PP1.

Purification and Purity: Protein A purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: PPP1CB polyclonal antibody detects endogenous levels of PPP1CB protein.

Bioworld Molecular Weight: 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PPP1CB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: A431 cell lysate
Lane 4: SH-SY-5Y cell lysate
Lane 5: SiHa cell lysate
Lane 6: 293 cell lysate
Lane 7: Hela cell lysate
Lane 8: PC-3M cell lysate
Lane 9: HepG2 cell lysate
Lane 10: A549 cell lysate, Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PPP1CB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-40 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Alternative Name: PP-1B, PPP1CD, PPP1CB, MGC3672, PP 1B, PP-1B, PP1B, PP1B, PP1beta, PPP1CB, PPP1CD, Protein phosphatase 1 beta, Protein phosphatase 1 catalytic subunit beta isoform, Protein phosphatase 1 delta, Protein phosphatase 1, catalytic subunit, beta isozyme, Protein phosphatase 1, catalytic subunit, delta isoform, Serine threonine protein phosphatase PP1 beta catalytic subunit, Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody

Immunogen: Recombinant protein corresponding to human PPP1CB aa 100-327.

Conjugate: Unconjugated

Modification: Unmodified