PKCβ Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91081-50, BS91081-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05771(Human) P68404(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC

All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200

Background: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Purification and Purity: Protein affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PKCβ polyclonal antibody detects endogenous levels of PKCβ protein.

Bioworld Molecular Weight: 77 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PKC beta 2 on different lysates using anti-PKC beta 2 antibody at 1/500 dilution.
Positive control:
Lane 1: Rat spleen tissue
Lane 2: Jurkat, ICC staining PKC beta 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PKC-B, PRKCB, PKC-beta, PKCB, PRKCB1, KPCB, PKC beta, PKC-B, PKC-beta, PKCB, Prkcb, PRKCB I, PRKCB1, PRKCB2, Protein kinase C beta, Protein kinase C beta type, protein kinase C, beta 1 polypeptide, protein kinase C, beta-1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified