IP6K2 (L188) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2759-50, BS2759-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UHH9

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The members of the inositol hexakisphosphate kinase family, IP6K1 and IP6K2, have a high affinity and selectivity for inositol hexakisphosphate (InsP6) as a substrate. IP6K1 and IP6K2 (also designated PiUS) convert InsP6 to PP-InsP5. However, neither kinase demonstrates any catalytic activity with other inositol pyrophosphates. The presence of InsP6, which inhibits serine/threonine protein phosphatases, increases the influx of calcium across the plasma membrane and implies that it may mediate the regulation of insulin exocytosis. IP6K1 was purified as a 54 kDa protein in rat brain extracts. By homology, IP6K1 and IP6K2 were characterized in mouse as a 50 kDa and 49 kDa protein, respectively. IP6K1 displays ATP synthase activity by transferring a phosphate from PP-InsP5 to ADP, which suggests a role for the IP6 kinases as high energy phosphate donors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IP6K2 (L188) polyclonal antibody detects endogenous levels of IP6K2 protein.

Bioworld Molecular Weight: ~ 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IP6K2 (L188) pAb at 1:500 dilution
Lane 1: CT26 whole cell lysate (40ug)
Lane 2: C6 whole cell lysate (40ug)
Lane 3: Panc1 whole cell lysate (40ug)
Lane 4: HCT116 whole cell lysate (40ug)
Lane 5: A549 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of IP6K2 (L188) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of IP6K2 (L188) pAb in paraffin-embedded human stomach carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Inositol hexakisphosphate kinase 2; InsP6 kinase 2; P(i)-uptake stimulator; PiUS; IP6K2; IHPK2; TCCCIA00113

Immunogen: Synthetic peptide, corresponding to amino acids 156-204 of Human IP6K2.

Conjugate: Unconjugated

Modification: Unmodified