NRF1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90968-50, BS90968-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16656(Human) Q9WU00(Mouse) Q62792(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC, IP,

All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NRF1 polyclonal antibody detects endogenous levels of NRF1 protein.

Bioworld Molecular Weight: 56 kDa/65kDa(Observed)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NRF1 on Hela cell (1) and mouse heart tissue (2) lysate using anti-NRF1 antibody at 1/500 dilution., ICC staining NRF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: NRF-1, Alpha-pal, NRF1, alpha pal, alpha palindromic binding protein, Alpha palindromic-binding protein, Alpha-pal, locus control region factor 1, NFE2 related factor 1, NRF-1, Nrf1, NRF1, Nuclear respiratory factor 1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified