NSD3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90970-50, BS90970-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BZ95(Human) Q6P2L6(Mouse) EntrezGene:290831(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100

Background: The deduced 1,437 amino acid NSD3 protein contains two PWWP domains involved in protein-protein interactions, five PHD-type zinc finger motifs found in chromatin-associated proteins, a SAC (SET-associated cys-rich) domain, a SET domain and a C-terminal C5HCH domain. Two NSD3 variants have been identified. The short variant comprised of 645 amino acids, arises from alternative polyadenylation and exon splicing and contains a single PWWP domain. A longer NSD3 variant, which is only expressed in HeLa cells, is comprised of 1,388 amino acid residues. The human WHSC1L1 gene, which encodes the NSD3 protein, shares 68% and 55% identity with mouse Nsd1 and human WHSC1, respectively. Highest expression of NSD3 is observed in brain, heart and skeletal muscle tissues; lower levels of NSD3 expression are observed in the liver and lungs.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NSD3 polyclonal antibody detects endogenous levels of NSD3 protein.

Bioworld Molecular Weight: 162 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: 293T, Flow cytometric analysis of Hela cells with NSD3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Alternative Name: WHSC1-like protein 1, WHSC1L1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, Histone-lysine N-methyltransferase NSD3, MGC126766, MGC142029, NSD 3, NSD3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, Nuclear SET domain-containing protein 3, pp14328, Protein whistle, Whistle, WHSC1 like protein 1, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, WHSC1-like protein 1, WHSC1L1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1, Wolf-Hirschhorn syndrome candidate 1-like protein 1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified